Introduction. Nowadays the issue of professional burnout is one of the most important. Especially stressful professions are those in which employees interact with people, i.e. professions of the category ‘human-to-human’, this criterion concerns medical personnel. The aim of the work is to investigate the possibilities of preventing emotional and professional burnout through stress prevention. Materials and methods. The sample consisted of medical staff of the regional centre of palliative care for children ‘Children’s hospice’ with a total of 40 people. The study was conducted by means of the following methods: psychological stress scale PSM-25, express-diagnosis of the level of personal frustration (V.V. Boyko), questionnaire of coping behaviour R. Lazarus, questionnaire of professional ‘burnout’ (K. Maslach, S. Jackson, adapted by N.E. Vodopyanova). The T-Wilcoxon test was used to identify differences. Results. According to the method ‘Psychological Stress Scale PSM-25’ p=0,007, T=91. The method ‘Express-diagnostics of the level of personal frustration (V.V. Boyko)’ p=0,03, T=108. According to the technique ‘Questionnaire of ways of coping behaviour by R. Lazarus’ self-control - p=0,0004, T=29; escape and avoidance - p=0,0001, T=27; planning of the problem solution - p=0,0007, T=91; positive reassessment - p=0,0007, T=52. According to the method ‘Professional Burnout Questionnaire (K. Maslach, S. Jackson, adapted by N.E. Vodopyanova)’ of psychoemotional exhaustion - the significance level was p=0.00002, T=38, and depersonalisation - the significance level was p=0.0007, T=52. The level of significance of burnout in general was p=0.002, T=108. Discussion. Based on the results, the formation of stress tolerance in medical staff is able to level the level of professional burnout formation. Conclusion. Stress tolerance plays an important role in adapting to stressors at work.